Journal: Scientific reports

Article Title: Potential in vitro anti-periodontopathogenic, anti-Chikungunya activities and in vivo toxicity of Brazilian red propolis.

doi: 10.1038/s41598-022-24776-4

Figure Lengend Snippet: Figure 2. Antibiofilm activity of Brazilian Red Propolis crude hydroalcoholic extract samples and number of viable cells in monospecies biofilms formed by ATCC strains and clinical isolates included in the study. (a) P. gingivalis (ATCC 49417). (b) P. gingivalis (clinical isolate). (c) P. intermedia (ATCC 15033). (d) P. intermedia (clinical isolate). (e) A. naeslundii (ATCC 19039). (f) F. nucleatum (clinical isolate).

Article Snippet: The tested bacteria were periodontal F. nucleatum (ATCC 10953), P. gingivalis (ATCC 33277), and P. intermedia (ATCC 25611).

Techniques: Activity Assay

Journal: Scientific reports

Article Title: Potential in vitro anti-periodontopathogenic, anti-Chikungunya activities and in vivo toxicity of Brazilian red propolis.

doi: 10.1038/s41598-022-24776-4

Figure Lengend Snippet: Figure 3. Antibiofilm activity of oblongifolin B and number of viable cells in monospecies biofilms formed by ATCC strains and clinical isolates included in the study. (a) P. intermedia (clinical isolate) (b) P. intermedia (ATCC 15033). (c) A. naeslundii (ATCC 19039). (d) F. nucleatum (clinical isolate).

Article Snippet: The tested bacteria were periodontal F. nucleatum (ATCC 10953), P. gingivalis (ATCC 33277), and P. intermedia (ATCC 25611).

Techniques: Activity Assay

Journal: Scientific reports

Article Title: Potential in vitro anti-periodontopathogenic, anti-Chikungunya activities and in vivo toxicity of Brazilian red propolis.

doi: 10.1038/s41598-022-24776-4

Figure Lengend Snippet: Figure 4. Antibiofilm activity of guttiferone E and number of viable cells in monospecies biofilms formed by ATCC strains and clinical isolates included in the study. (a) P. gingivalis (ATCC 49417). (b) P. gingivalis (clinical isolate). (c) P. intermedia (ATCC 15033). (d) A. naeslundii (ATCC 19039). (e) F. nucleatum (clinical isolate).

Article Snippet: The tested bacteria were periodontal F. nucleatum (ATCC 10953), P. gingivalis (ATCC 33277), and P. intermedia (ATCC 25611).

Techniques: Activity Assay

Journal:

Article Title: Native Plasmids of Fusobacterium nucleatum : Characterization and Use in Development of Genetic Systems †

doi:

Figure Lengend Snippet: Relevant characteristics of the bacterial strains and plasmids used in this study

Article Snippet: The strain harboring pFN3, ATCC 10953, was previously reported to lack plasmid DNA ( 20 ).

Techniques: Plasmid Preparation, Cloning

Journal:

Article Title: Native Plasmids of Fusobacterium nucleatum : Characterization and Use in Development of Genetic Systems †

doi:

Figure Lengend Snippet: Restriction maps and Southern blots of the F. nucleatum plasmids. (A) Partial restriction map of F. nucleatum plasmids pFN1, pFN2 and pFN3 and shuttle plasmid pHS17. Selected restriction endonuclease sites in the native plasmids are presented. Restriction endonuclease sites indicated for pHS17 relate to the plasmid construction. The pFN1 portion of pHS17 is indicated by the thick solid bar, with the position of the repA homologue (ORF5) and putative ori indicated. (B and C) Plasmids from F. nucleatum strains 12230 (pFN1, lanes 1), 10113 (pFN2, lanes 2), and ATCC 10953 (pFN3, lanes 3) were probed with pFN1 DNA with EcoRI digests (B) or pFN3 DNA with EcoRV digests (C). The HincII-digested pFN1 and AseI-digested pFN3 probes were 32P labeled (specific activity of 25 × 108 and 4.5 × 108 dpm/μg of DNA, respectively). The positions of molecular size markers are indicated on the left, and the linear forms of pFN1, pFN2, and pFN3 are indicated on the right.

Article Snippet: The strain harboring pFN3, ATCC 10953, was previously reported to lack plasmid DNA ( 20 ).

Techniques: Plasmid Preparation, Labeling, Activity Assay

Journal:

Article Title: Native Plasmids of Fusobacterium nucleatum : Characterization and Use in Development of Genetic Systems †

doi:

Figure Lengend Snippet: Plasmid DNA from F. nucleatum ATCC 10953 transformants consists of the shuttle plasmid, pHS17, and the native plasmid, pFN3. Plasmid preparations from E. coli (pHS17), F. nucleatum ATCC 10953 transformant strain KH21 (pHS17 and pFN3), and F. nucleatum ATCC 10953 (pFN3) were analyzed. The preparations were either not digested (lanes 1) or predigested with EcoRV (lanes 2) or EcoRI (lanes 3), separated on 0.8% agarose gels, stained with ethidium bromide, and visualized under UV illumination. The open circular (OC), linear (L), and covalently closed circular (CC) forms of pHS17 and pFN3 are indicated on the left and right, respectively.

Article Snippet: The strain harboring pFN3, ATCC 10953, was previously reported to lack plasmid DNA ( 20 ).

Techniques: Plasmid Preparation, Staining